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aroma.affymetrix (version 1.4.0)

Analysis of large Affymetrix microarray data sets

Description

This package implements classes for files and sets of files for various Affymetrix file formats, e.g. AffymetrixCdfFile, AffymetrixCelFile, and AffymetrixCelSet. These are designed to be memory efficient but still being fast. The idea is to keep all data on file and only read data into memory when needed. Clever caching mechanisms are used to minimize the overhead of data IO. All of the above is hidden in the package API and for the developer (and the end user), the data is queried as if it lives in memory. With this design it is only the diskspace that limits what number of arrays can be analyzed.

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Version

Install

install.packages('aroma.affymetrix')

Monthly Downloads

719

Version

1.4.0

License

LGPL (>= 2.1)

Maintainer

Henrik Bengtsson

Last Published

January 7th, 2010

Functions in aroma.affymetrix (1.4.0)

AffymetrixFile

The abstract AffymetrixFile class
ChipEffectGroupMerge

The ChipEffectGroupMerge class
CnChipEffectFile

The CnChipEffectFile class
ChipEffectSet

The ChipEffectSet class
CnagCfhSet

The CnagCfhSet class
ExonChipEffectSet

The ExonChipEffectSet class
AlleleSummation

The AlleleSummation class
AffymetrixCdfFile

The AffymetrixCdfFile class
QuantileNormalization

The QuantileNormalization class
AbstractProbeSequenceNormalization

The AbstractProbeSequenceNormalization class
AffineCnPlm

The AffineCnPlm class
FragmentEquivalentClassNormalization

The FragmentEquivalentClassNormalization class
AffinePlm

The AffinePlm class
CnagCfhFile

The CnagCfhFile class
SnpInformation

The SnpInformation class
WeightsFile

The WeightsFile class
bgAdjustGcrma.AffymetrixCelSet

Applies probe sequence based background correction to a set of CEL files
CnPlm

The CnPlm class
CrlmmParametersSet

The CrlmmParametersSet class
RmaCnPlm

The RmaCnPlm class
DChipDcpSet

The DChipDcpSet class
fit.Model

Estimates the model parameters
GenomeInformation

The GenomeInformation class
HetLogAddSnpPlm

The HetLogAddSnpPlm class
MatSmoothing

The MatSmoothing class
Model

The Model class
ScaleNormalization

The ScaleNormalization class
SnpChipEffectFile

The SnpChipEffectFile class
MultiArrayUnitModel

The MultiArrayUnitModel class
AvgSnpPlm

The AvgSnpPlm class
QualityAssessmentSet

The QualityAssessmentSet class
ParameterCelSet

The ParameterCelSet class
DChipSnpInformation

The DChipSnpInformation class
as.AffymetrixCelSet.AffymetrixCelSet

Coerce an object to an AffymetrixCelSet object
BaseCountNormalization

The BaseCountNormalization class
SmoothRmaModel

The SmoothRmaModel class
AffymetrixCelFile

The AffymetrixCelFile class
FirmaModel

The FirmaModel class
allocateFromCdf.AffymetrixCelFile

Creates an empty CEL file from a template CDF
createUniqueCdf.AffymetrixCdfFile

Creates a unique-cell version of the CDF
AffymetrixFileSetReporter

The AffymetrixFileSetReporter class
byChipType.AromaChipTypeAnnotationFile

Defines an AromaChipTypeAnnotationFile object by chip type
clearData.AffymetrixCelFile

Clears all or a subset of the fields in a CEL file
SnpChipEffectSet

The SnpChipEffectSet class
LimmaBackgroundCorrection

The LimmaBackgroundCorrection class
byChipType.DChipSnpInformation

Defines a DChipSnpInformation object by chip type
getChipEffectSet.AlleleSummation

Gets the set of chip effects for this model
fit.SingleArrayUnitModel

Estimates the model parameters
as.AffymetrixCnChpSet.AffymetrixCnChpSet

Coerce an object to an AffymetrixCnChpSet object
RmaPlm

The RmaPlm class
AffymetrixFileSet

The AffymetrixFileSet class
AdditiveCovariatesNormalization

The AdditiveCovariatesNormalization class
AffineSnpPlm

The AffineSnpPlm class
SpatialReporter

The SpatialReporter class
SnpProbeAffinityFile

The SnpProbeAffinityFile class
UnitTypeScaleNormalization

The UnitTypeScaleNormalization class
AffymetrixProbeTabFile

The AffymetrixProbeTabFile class
AffymetrixCnChpSet

The AffymetrixCnChpSet class
createMonocellCdf.AffymetrixCdfFile

Creates a mono-cell version of the CDF
LinearModelProbeSequenceNormalization

The LinearModelProbeSequenceNormalization class
averageQuantile.AffymetrixCelSet

Calculates the average empirical distribution across all samples
DChipDcpFile

The DChipDcpFile class
AllelicCrosstalkCalibration

The AllelicCrosstalkCalibration class
WeightsSet

The WeightsSet class
AvgPlm

The AvgPlm class
getAlleleProbePairs.AffymetrixCdfFile

Gets the indices of probepairs with the same pair of SNP nucleotides
as.DChipDcpSet.DChipDcpSet

Coerce an object to an DChipDcpSet object
findUnitsTodo.FirmaModel

Identifies non-fitted units
as.character.AffymetrixCelSet

Returns a short string describing the Affymetrix CEL set
getCnNames.AffymetrixCdfFile

Gets the names of the CN units
getTags.Model

Gets the tags of the output data set
compare.AffymetrixCdfFile

Checks if two AffymetrixCdfFile objects are equal
allocateFromCdf.AromaUnitTabularBinaryFile

Creates an AromaUnitTabularBinaryFile mapping to a given CDF
fromFile.AffymetrixCelFile

Defines an AffymetrixCelFile object from a CEL file
getAverageFile.CnagCfhSet

Calculates the mean and the standard deviation of the cell signal (intensity, standard deviation etc.) across the CFH set
isDuplicated.CnagCfhSet

Identifies duplicated CFH files
FirmaSet

The FirmaSet class
OpticalBackgroundCorrection

The OpticalBackgroundCorrection class
DChipGenomeInformation

The DChipGenomeInformation class
fit.FirmaModel

Estimates the model parameters
plotDensity.AffymetrixCelFile

Plots the density of the probe signals on the array
ChipEffectFile

The ChipEffectFile class
getData.GenomeInformation

Gets all or a subset of the genome information data
byPath.AffymetrixFileSet

Defines an AffymetrixFileSet object by searching for Affymetrix files
getCdf.CnagCfhFile

Gets the CDF structure for this CEL file
getAverageFile.AffymetrixCelSet

Calculates the mean and the standard deviation of the cell signal (intensity, standard deviation etc.) across the CEL set
CnProbeAffinityFile

The CnProbeAffinityFile class
FragmentLengthNormalization

The FragmentLengthNormalization class
getChipType.AffymetrixCelSet

Gets the chip type for this CEL set
fromFile.CnagCfhFile

Defines an CnagCfhFile object from a CFH file
fromFile.AromaChipTypeAnnotationFile

Sets up an AromaChipTypeAnnotationFile
findByChipType.AromaChipTypeAnnotationFile

Locates an annotation file by its chip type
findByChipType.AffymetrixCdfFile

Locates a CDF file from its chip type
getUnitIntensities.AffymetrixCelSet

Gets cell signals for a subset of units and a subset of arrays
HetLogAddPlm

The HetLogAddPlm class
ProbeLevelTransform3

The ProbeLevelTransform3 class
bgAdjustGcrma.AffymetrixCelFile

Applies probe sequence based background correction to a CEL file
QualityAssessmentFile

The QualityAssessmentFile class
getChipType.GenomeInformation

Gets the chip type of this genome information set
getChipType.AffymetrixCelFile

Gets the chip type for this CEL file
getData.SnpInformation

Gets all or a subset of the genome information data
getCdf.AffymetrixCelFile

Gets the CDF structure for this CEL file
getOutputDataSet.Transform

Gets the transformed data set
getAlias.GenericReporter

Gets the alias of the report
getFitUnitGroupFunction.SingleArrayUnitModel

Static method to get the low-level function that fits the PLM
UgpGenomeInformation

The UgpGenomeInformation class
getCdf.Model

Gets the CDF structure for this model
getFitUnitGroupFunction.ExonRmaPlm

Gets the low-level function that fits the Exon PLM
calculateBaseline.ChipEffectSet

Estimates the baseline signal chromosome by chromosome
getProbeAffinityFile.ProbeLevelModel

Gets the probe affinities for this model
RmaSnpPlm

The RmaSnpPlm class
bgAdjustRma.AffymetrixCelSet

Applies RMA background correction to a set of CEL files
HetLogAddCnPlm

The HetLogAddCnPlm class
process.ScaleNormalization

Normalizes the data set
getCellIndices.AffymetrixCdfFile

Gets the cell indices unit by unit
ReseqCrosstalkCalibration

The ReseqCrosstalkCalibration class
getFullName.Model

Gets the full name of the output set
DChipCdfBinFile

The DChipCdfBinFile class
AffymetrixCelSetReporter

The AffymetrixCelSetReporter class
AvgCnPlm

The AvgCnPlm class
readUnits.AffymetrixCelFile

Reads CEL data unit by unit
ChipEffectTransform

The ChipEffectTransform class
Non-documented objects

Non-documented objects
CrlmmParametersFile

The CrlmmParametersFile class
getName.Model

Gets the name of the output data set
getCdf.AffymetrixCelSet

Gets the CDF structure for this CEL set
BackgroundCorrection

The BackgroundCorrection class
getFitUnitGroupFunction.AffinePlm

Gets the low-level function that fits the PLM
ParameterCelFile

The ParameterCelFile class
getDataSet.Model

Gets the input data set for this model
getFirmaSet.FirmaModel

Gets the set of FIRMA results for this model
fromDataFile.ChipEffectFile

Retrive an existing CEL file, or create from CDF template if missing
calculateResidualSet.FirmaModel

Gets the set of residuals corresponding to the PLM
process.BackgroundCorrection

Processes the data set
findUnitsTodo.UnitModel

Identifies non-fitted units
plotDensity.AffymetrixCelSet

Plots the densities of all samples
ExonProbeAffinityFile

The ExonProbeAffinityFile class
getBaseline.ChipEffectSet

Gets the baseline signals across chromosomes
computeAffinities.AffymetrixCdfFile

Calculates probe affinities from sequence
process.MatSmoothing

Processes the data set
getInputDataSet.TransformReport

Gets the source data set
QualityAssessmentModel

The QualityAssessmentModel class
getIntensities.AffymetrixCelSet

Gets cell intensities from a set of cells and a set of arrays
getFullName.TransformReport

Gets the full name of the output data set
convertUnits.AffymetrixCdfFile

Gets and validates unit indices
getAlleleProbePairs3.AffymetrixCdfFile

Gets the indices of probepairs with the same pair of SNP nucleotides
fitQuantileNormFcn.AffymetrixCelFile

Fits quantile normalization functions for the arrays in the data set
getAlias.Model

Gets the name alias for the model
getChipEffectSet.ProbeLevelModel

Gets the set of chip effects for this model
getUnitTypes.AffymetrixCdfFile

Gets the types of a set of units
getUnitIndices.GenomeInformation

Gets unit indices ordered along the chromosome
process.LimmaBackgroundCorrection

Performs background correction
process.ReseqCrosstalkCalibration

Calibrates the data set
process.AbstractProbeSequenceNormalization

Normalizes the data set
GcRmaBackgroundCorrection

The GcRmaBackgroundCorrection class
ExonChipEffectFile

The ExonChipEffectFile class
bgAdjustRma.AffymetrixCelFile

Applies normExp background correction to a CEL file
setCdf.CnagCfhFile

Sets the CDF structure for this CEL file
process.FragmentEquivalentClassNormalization

Normalizes the data set
process.RmaBackgroundCorrection

Performs background correction
getCellIndices.UnitModel

Gets the cell indices unit by unit
getTags.GenericReporter

Gets the tags of the reporter
getSnpNames.AffymetrixCdfFile

Gets the names of the SNP units
getRootPath.Model

Gets the root path of this model
getUnitGroupCellMap.ChipEffectFile

Gets a (unit, group, cell) index map
process.ScaleNormalization3

Normalizes the data set
as.AffymetrixFileSet.AffymetrixFileSet

Coerce an object to an AffymetrixFileSet object
getXAM.ChipEffectFile

Gets the physical position, log-intensities and log-ratios of chip effects for two arrays
image270.AffymetrixCelFile

Displays all or a subset of the data spatially
CnChipEffectSet

The CnChipEffectSet class
process.UnitTypeScaleNormalization

Normalizes the data set
isResequenceChip.AffymetrixCdfFile

Static method to check if a CDF is for a resequencing (CustomSeq) chip
nbrOfArrays.DChipDcpSet

Gets the number of arrays in the file set
AromaChipTypeAnnotationFile

The AromaChipTypeAnnotationFile class
ScaleNormalization3

The ScaleNormalization3 class
bgAdjustOptical.AffymetrixCelSet

Applies optical background correction to a set of CEL files
getPositions.GenomeInformation

Gets the physical positions for a set of units
ExonRmaPlm

The ExonRmaPlm class
plotImage.AffymetrixCelFile

Displays a spatial plot of a CEL file
process.FragmentLengthNormalization

Normalizes the data set
setArrays.ArrayExplorer

Sets the arrays
readUnits.CnagCfhFile

Reads CEL data unit by unit
nbrOfSnps.AffymetrixCdfFile

Gets the number of SNPs
UnitModel

The UnitModel class
NormExpBackgroundCorrection

The NormExpBackgroundCorrection class
MbeiSnpPlm

The MbeiSnpPlm class
readUnits.SingleArrayUnitModel

Reads data unit by unit
setRestructor.AffymetrixCdfFile

Specifies a function through which
calculateParametersGsb.AffymetrixCelSet

Computes parameters for adjustment of specific binding
fit.ProbeLevelModel

Estimates the model parameters
process.OpticalBackgroundCorrection

Performs background correction
setAlias.GenericReporter

Sets the alias of the report
getHeader.AffymetrixCdfFile

Gets the header of the CDF file
setAlias.Model

Sets the name alias for the model
MbeiCnPlm

The MbeiCnPlm class
SingleArrayUnitModel

The SingleArrayUnitModel class
getXAM.ChipEffectSet

Gets the physical position, log-intensities and log-ratios of chip effects for two arrays
readUnitsByQuartets.AffymetrixCdfFile

Gets the cell quartets for each base position
getAM.ChipEffectFile

Gets the log-intensities and log-ratios of chip effects for two arrays
ProbeLevelModel

The ProbeLevelModel class
ResidualSet

The ResidualSet class
DChipQuantileNormalization

The DChipQuantileNormalization class
ResidualFile

The ResidualFile class
getGenomeInformation.AffymetrixCdfFile

Gets genome information for this chip type
normalizeQuantile.AffymetrixCelSet

Normalizes samples to have the same empirical distribution
AffymetrixCelSetTuple

The AffymetrixCelSetTuple class
process.ArrayExplorer

Generates image files, scripts and dynamic pages for the explorer
readUnits.MultiArrayUnitModel

Reads data unit by unit
nbrOfGroupsPerUnit.AffymetrixCdfFile

Gets the number of groups in each unit
extractMatrix.AffymetrixCelSet

Extract data as a matrix for a set of arrays
process.QuantileNormalization

Normalizes the data set
importFromApt.CnChipEffectSet

Imports copy-number estimates from an APT summary file
process.GcContentNormalization

Normalizes the data set
verify.GenomeInformation

Verifies the correctness of the underlying file
setCdf.AffymetrixCelSet

Sets the CDF structure for this CEL set
process.MatNormalization

Normalizes the data set
bgAdjustOptical.AffymetrixCelFile

Applies optical background correction to a CEL file
byChipType.DChipGenomeInformation

Defines a DChipGenomeInformation object by chip type
extractMatrix.ParameterCelSet

Extract data as a matrix for a set of arrays
fromCdf.SnpInformation

Static method to define a genome information set from a CDF
readCdfDataFrame

Reads units (probesets) from an Affymetrix CDF file
GenericReporter

The GenericReporter class
writeImage.AffymetrixCelFile

Writes a spatial image of the signals in the CEL file
setCdf.CnagCfhSet

Sets the CDF structure for this CFH set
getFitUnitGroupFunction.HetLogAddPlm

Gets the low-level function that fits the PLM
getFitUnitGroupFunction.AvgPlm

Gets the low-level function that fits the PLM
byChipType.GenomeInformation

Static method to define a genome information set by chip type
getHeader.AffymetrixCelFile

Gets the header of the CEL file
MbeiPlm

The MbeiPlm class
readUnits.AffymetrixCdfFile

Reads CDF data unit by unit
setCdf.AffymetrixCelFile

Sets the CDF structure for this CEL file
findUnitsTodo.ProbeLevelModel

Identifies non-fitted units
ProbeAffinityFile

The ProbeAffinityFile class
as.CnagCfhSet.CnagCfhSet

Coerce an object to an CnagCfhSet object
verify.SnpInformation

Verifies the correctness of the underlying file
fit.SmoothMultiarrayModel

Fits the model for one chromosome across samples
importFromDChip.AffymetrixCelSet

Imports dChip-exported CEL files
updateUnits.AffymetrixCelFile

Updates CEL data unit by unit
getName.TransformReport

Gets the name of the output data set
getDataSet.AffymetrixCelSetReporter

Gets the data set
getFitUnitGroupFunction.MbeiPlm

Gets the low-level function that fits the PLM
plotMvsA.AffymetrixCelFile

Plots log-ratio versus log-intensity in a scatter plot
isDuplicated.AffymetrixCelSet

Identifies duplicated CEL files
getFitUnitGroupFunction.FirmaModel

Static method to get the low-level function that fits the PLM
aroma.affymetrix-package

Package aroma.affymetrix
getTags.TransformReport

Gets the tags of the output data set
as.character.CnagCfhSet

Returns a short string describing the CNAG CFH set
as.character.DChipDcpSet

Returns a short string describing the DChip CHP set
fromCdf.GenomeInformation

Static method to define a genome information set from a CDF
getAM.ChipEffectSet

Gets the log-intensities and log-ratios of chip effects of the set relative to a reference chip effect file
importFromDChip.CnChipEffectSet

Imports copy-number estimates from a dChip result file
getPath.Model

Gets the path of this model
getHeader.AromaChipTypeAnnotationFile

Gets the header of the annotation file
nbrOfArrays.AffymetrixCelSet

Gets the number of arrays in the file set
smoothScatterMvsA.AffymetrixCelFile

Plots log-ratio versus log-intensity in a smooth scatter plot
process.SpatialReporter

Generates image files, scripts and dynamic pages for the explorer
getImage.AffymetrixCelFile

Creates an RGB Image object from a CEL file
getTimestamp.AffymetrixCelFile

Gets the timestamp in the CEL header
getAlleleCellPairs.AffymetrixCdfFile

Gets the cell indices of allele pairs
getResiduals.QualityAssessmentModel

Calculates the residuals from a probe-level model
getWeights.QualityAssessmentModel

Calculates the weights from the robust fit to a probe-level model
isPm.AffymetrixCdfFile

Checks which cells (probes) are PMs and not
getHeader.CnagCfhFile

Gets the header of the CEL file
getPath.TransformReport

Gets the path of the output data set
nbrOfArrays.CnagCfhSet

Gets the number of arrays in the file set
process.ChipEffectGroupMerge

Normalizes the data set
isSnpChip.AffymetrixCdfFile

Static method to check if a chip is a mapping (SNP) chip
plotBoxplotStats.list

Plots a (merged or non-merged) list of boxplot.stats() elements
plotMvsX.AffymetrixCelFile

Plots log-ratio versus another variable in a scatter plot
process.AdditiveCovariatesNormalization

Normalizes the data set
process.GcRmaBackgroundCorrection

Performs background correction
AffymetrixCelSet

The AffymetrixCelSet class
ArrayExplorer

The ArrayExplorer class
BasePositionNormalization

The BasePositionNormalization class
MatNormalization

The MatNormalization class
SnpChipEffectGroupMerge

The SnpChipEffectGroupMerge class
TransformReport

The TransformReport class
as.character.AffymetrixCnChpSet

Returns a short string describing the set
byChipType.SnpInformation

Static method to define a genome information set by chip type
extractDataFrame.ParameterCelSet

Extract data as a data.frame for a set of arrays
extractAffyBatch.AffymetrixCelSet

Extracts an in-memory AffyBatch object from the CEL set
createFrom.AffymetrixCelFile

Creates a CEL file using another as a template
getCdf.CnagCfhSet

Gets the CDF structure for this CFH set
getFitUnitGroupFunction.MultiArrayUnitModel

Static method to get the low-level function that fits the PLM
normalizeQuantile.AffymetrixCelFile

Normalizes the probe intensities to a target empirical distribution
plotDensity.GenomeInformation

Plots the density of SNPs for a given chromosome
setTags.Model

Sets the tags to be appended
GcContentNormalization2

The GcContentNormalization2 class
GcContentNormalization

The GcContentNormalization class
RmaBackgroundCorrection

The RmaBackgroundCorrection class
SmoothMultiarrayModel

The SmoothMultiarrayModel class
SnpPlm

The SnpPlm interface class
convert.AffymetrixCdfFile

Converts a CDF into the same CDF but with another format
fromFile.AffymetrixCdfFile

Defines an AffymetrixCdfFile object from a CDF file
getChipType.SnpInformation

Gets the chip type of this genome information set
getDataSet.ArrayExplorer

Gets the data set
getName.GenericReporter

Gets the name of the explorer
getOutputDataSet.TransformReport

Gets the transformed data set
process.AllelicCrosstalkCalibration

Calibrates the data set
process.DChipQuantileNormalization

Normalizes the data set
FirmaFile

The FirmaFile class
ProbeLevelTransform

The ProbeLevelTransform class
byChipType.UgpGenomeInformation

Defines a UgpGenomeInformation object by chip type
getChipType.CnagCfhFile

Gets the chip type for this CEL file
getFitUnitGroupFunction.RmaPlm

Gets the low-level function that fits the PLM
getUnitNames.AffymetrixCdfFile

Gets the names of each unit
nbrOfArrays.AffymetrixCnChpSet

Gets the number of arrays in the file set
process.GenericReporter

Generates report
readRawData.AffymetrixCelFile

Gets all or a subset of the fields in a CEL file