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Data set of a melting curve using the Bio-Rad iQ5 thermo cycler. The samples of Vimentin were measured in the CFX96 as replicates according to Roediger et al. (2013). The quantification was performed during the gradient from 55 to 95 degrees Celsius with a resolution of 0.5 degrees Celsius per step.
data(VIMiQ5_melt)
A data frame with 81 observations on the following 97 variables. The first column ("T") contains the temperature and consecutive columns contain the replicates ("A01" to "H12").
A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:33--74, 2013.
# NOT RUN {
data(VIMiQ5_melt)
tmp <- VIMiQ5_melt
plot(NA, NA, xlim = c(55,95), ylim = c(0, 40000),
xlab = "Temperature (degrees Celsius)",ylab = "RFU",
main = "Melting curve in iQ5 (Bio-Rad)")
apply(tmp[, 2:ncol(tmp)], 2,
function(x) lines(tmp[1:nrow(tmp),1],x))
Fmean <- rowMeans(tmp[, 2:ncol(tmp)])
lines(tmp[1:nrow(tmp),1], Fmean, col = "red", lwd = 3)
legend(55, 4000, c("Raw", "Mean"), pch = c(19,19), col = c(1,2))
# }
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