call_targets

The minimum posterior probability of a droplet to be 
classified as a cell/nucleus.

pp_thresh

The minimum number of genes a droplet must have 
to be classified as a cell/nucleus.

min_genes

Call cells or nuclei from an SCE object. EM must be run beforehand.
The posterior probability is defined as 1 - Prob(Debris), where 
Prob(Debris) is the posterior probability that a droplet is 
debris.

Identification of debris-containing droplets
from a droplet-based single cell/nucleus RNA-seq. 'diem' uses
the expression profile of background RNA droplets, followed
by expectation maximization (EM), to assign candidates as
debris or nuclei. See Alvarez M, Rahmani E, et al (2019)
<doi:10.1101/786285> for details.

call_targets: Call clean droplets after running EM

Description

Usage

Arguments

Value