computeLogRatio: Summary statistics for gene expression

Description

Compute summary statistics per gene of expression data in a ExpressionSet object.

Usage

computeLogRatio(e, reference, within = NULL, across = NULL, nReplicatesVar = 3, ...)

Arguments

An object of class ExpressionSet

reference

A list with two items: var and level - See details

within

Character vector - names of pData columns - See details

across

Character vector - names of pData columns - See details

nReplicatesVar

Integer - Minimum number of replicates to compute variances

...

Value

Returns an object of class ExpressionSet with pData inherited from the submitted ExpressionSet object, supplemented by the computed statistics in the 'exprs' slot and info thereof in the 'phenoData' slot.

Details

Summary statistics (mean, variances and difference to reference or control) will be computed on the 'exprs' slot of the ExpressionSet object. The parameters of the computation are specified by the parameters 'reference', 'within' and 'across'.\ The design of the computations is such that the differences and pooled variances are calculated against the sample(s) that was(were) chosen as reference. The reference is specified by the level of a certain variable in the phenoData slot (e.g.: column 'control' and level 'WT' of the phenoData slot or a boolean ('ref') variable with 0 or 1) -- the list object of 'var' and 'level' together determine the reference group. \ All groups determined by combining the reference$var and across variables will be compared to the reference group. Two different approaches to obtain necessary computations:

{Prepare a boolean variable that reflects only the reference group and specify all groupings in the across arguments. E.g.:

reference=list(var = 'boolean', level = 1), 
       across = c('compound','dose')

} {Add an extra column to the phenoData slot that contains all combinations, with a specific one for the reference group: for example, pData(e)['refvar'] <- paste(pData(e)['compound'], pData(e)['dose'],sep='.') so as to use reference = list(var = 'refvar', level ='comp1.dose1') as argument for reference.}

Examples

Run this code

if (require(ALL)){
data(ALL, package = "ALL")
ALL <- addGeneInfo(ALL)
ALL$BTtype <- as.factor(substr(ALL$BT,0,1))
ALL2 <- ALL[,ALL$BT != 'T1']  # omit subtype T1 as it only contains one sample
ALL2$BTtype <- as.factor(substr(ALL2$BT,0,1)) # create a vector with only T and B

# Test for differential expression between B and T cells
tTestResult <- tTest(ALL, "BTtype", probe2gene = FALSE)
topGenes <- rownames(tTestResult)[1:20]

# plot the log ratios versus subtype B of the top genes 
LogRatioALL <- computeLogRatio(ALL2, reference=list(var='BT',level='B'))
a <- plotLogRatio(e=LogRatioALL[topGenes,],openFile=FALSE, tooltipvalues=FALSE, device='X11',
		colorsColumnsBy=c('BTtype'), main = 'Top 20 genes most differentially between T- and B-cells',
		orderBy = list(rows = "hclust"),
		probe2gene = TRUE)
}

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