Based on the Cleave-and-Rescue system of Oberhofer,
this is a 2-locus Cas9-based toxin-antidote system. The first locus carries the
Cas9, gRNAs, and a recoded copy of an essential gene. The second locus is the
targeted essential gene. This gene can be completely haplosufficient (hSuf = 1)
or completely haploinsufficient (hSuf = 0). It is assumed that having
2 copies of the gene (be it wild-type at the second locus or recoded at the first)
confers complete viability.
cubeClvR(
cF = 1,
crF = 0,
ccF = cF,
ccrF = crF,
cM = 1,
crM = 0,
ccM = cM,
ccrM = crM,
dW = 0,
drW = 0,
ddW = dW,
ddrW = drW,
hSuf = 1,
eta = NULL,
phi = NULL,
omega = NULL,
xiF = NULL,
xiM = NULL,
s = NULL
)Female cutting rate, one ClvR allele
Female functional resistance rate, one ClvR allele
Female cutting rate, two ClvR alleles
Female functional resistance rate, two ClvR alleles
Male cutting rate, one ClvR allele
Male functional resistance rate, one ClvR allele
Male cutting rate, two ClvR alleles
Male functional resistance rate, two ClvR alleles
Female deposition cutting rate
Female deposition functional resistance rate
Female deposition (HH) cutting rate
Female deposition (HH) functional resistance rate
Haplosufficiency level, default is completely sufficient
Genotype-specific mating fitness
Genotype-specific sex ratio at emergence
Genotype-specific multiplicative modifier of adult mortality
Genotype-specific female pupatory success
Genotype-specific male pupatory success
Genotype-specific fractional reduction(increase) in fertility
Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.