DiffBind (version 2.0.2)

dba.plotVenn: Draw 2-way, 3-way, or 4-way Venn diagrams of overlaps

Description

Draws 2-way, 3-way, or 4-way Venn diagrams of overlaps

Usage

dba.plotVenn(DBA, mask, overlaps, label1, label2, label3, label4, main, sub,  contrast, method=DBA$config$AnalysisMethod,  th=DBA$config$th, bUsePval=DBA$config$bUsePval, bDB=TRUE, bNotDB, bAll=TRUE, bGain=FALSE, bLoss=FALSE, labelAttributes, DataType=DBA$config$DataType)

Arguments

DBA

DBA object; if present, only the mask parameter will apply.

mask

mask or vector of peakset numbers indicating which peaksets to include in Venn diagram. Only 2 or 3 peaksets should be included. See dba.mask. Only one of mask or overlaps is used.

overlaps

overlap record, as computed by dba.overlap(Report=DBA_OLAP_PEAKS). Only one of mask or overlaps is used.

label1

label for first peakset in diagram

label2

label for second peakset in diagram

label3

label for third peakset in diagram

label4

label for fourth peakset in diagram

main

main title for plot

sub

subtitle for plot

contrast

contrast number(s) to use for results-based plots. This can be a vector of contrast numbers. See dba.show(DBA, bContrast=T) to get contrast numbers.

method

if contrast is specified, include results from analyses using this method or methods:

• DBA_DESEQ2
• DBA_DESEQ2_BLOCK
• DBA_EDGER
• DBA_EDGER_BLOCK
• DBA_ALL_METHODS
• DBA_ALL_BLOCK
• DBA_ALL_METHODS_BLOCK

th

if contrast is specified, use this significance threshold; all sites with FDR (or p-values, see bUsePval) less than or equal to this value will be considered differentially bound (DB).

bUsePval

if contrast is specified, this logical indicates whether to use FDR (FALSE) or p-value (TRUE) for thresholding.

bDB

if contrast is specified, this logical indicates that peaksets should include Differentially Bound (DB) sites (respecting the th, bUsePval, and fold parameters).

bNotDB

if contrast is specified, this logical indicates that peaksets should include non-Differentially Bound (non-DB) sites (respecting the th, bUsePval, and fold parameters).

bAll

if contrast is specified, this logical indicates peaksets combining peaks with both positive and negative fold changes should be included.

bGain

if contrast is specified, this logical indicates that peaksets with only positive fold changes should be included.

bLoss

if contrast is specified, this logical indicates that peaksets with only negative fold changes should be included.

labelAttributes

is labels are not specified, use these attributes to create default labels:

• DBA_ID
• DBA_TISSUE
• DBA_FACTOR
• DBA_CONDITION
• DBA_TREATMENT
• DBA_REPLICATE
• DBA_CONSENSUS
• DBA_CALLER

Only specified attributes that differ between peaksets will be used for labels; the ones that have the same value for all peaksets will be used as the default subtitle.

DataType

if bReturnPeaksets is set to TRUE, the class of object that peaksets should be returned as:

• DBA_DATA_GRANGES
• DBA_DATA_RANGEDDATA
• DBA_DATA_FRAME

Can be set as default behavior by setting DBA$config$DataType.

Alternatively, this can be set to:

• DBA_DATA_DBAOBJECT

to return a results-based DBA object, if a contrast is specified.

Value

Either a list of peaksets is returned invisibly (as decribed in dba.overlap), or, if DataType=DBA_DATA_DBAOBJECT, a results-based DBA object.

See Also

dba.analyze, dba.overlap, dba.report, dba.plotPCA, vennPlot

Examples

data(tamoxifen_peaks)

par(mfrow=c(2,2))
# 2-way Venn
dba.plotVenn(tamoxifen,6:7)
dba.plotVenn(tamoxifen,tamoxifen$masks$ZR75)

# 3-way Venn (done two different ways)
dba.plotVenn(tamoxifen,tamoxifen$masks$MCF7&tamoxifen$masks$Responsive)
olaps <- dba.overlap(tamoxifen,tamoxifen$masks$MCF7&tamoxifen$masks$Responsive)
dba.plotVenn(tamoxifen,overlaps=olaps,
             label1="Rep 1",label2="Rep 2",label3="Rep 3",main="MCF7 (Responsive) Replicates")

#Venn of overlaps
Responsive=dba(tamoxifen,tamoxifen$masks$Responsive)
Responsive
Responsive <- dba.peakset(Responsive,1:3,sampID="MCF7")
Responsive <- dba.peakset(Responsive,4:5,sampID="T47D")
Responsive <- dba.peakset(Responsive,6:7,sampID="ZR75")
par(mfrow=c(1,1))
dba.plotVenn(Responsive,Responsive$masks$Consensus)

#4-way overlap
data(tamoxifen_peaks)
tamoxifen <- dba.peakset(tamoxifen, consensus=DBA_TISSUE)
par(mfrow=c(1,1))
dba.plotVenn(tamoxifen,tamoxifen$masks$Consensus,main="Tissue consensus overlaps")

#Venns of differentially bound sites
data(tamoxifen_analysis)
tamoxifen <- dba.contrast(tamoxifen,categories=DBA_CONDITION,block=tamoxifen$masks$MCF7)
tamoxifen <- dba.analyze(tamoxifen,method=c(DBA_EDGER,DBA_DESEQ2))
dba.plotVenn(tamoxifen,contrast=1,method=DBA_ALL_METHODS_BLOCK)
dba.plotVenn(tamoxifen,contrast=1,method=DBA_ALL_BLOCK,bAll=FALSE,bGain=TRUE,bLoss=TRUE)
par(mfrow=c(2,1))
dba.plotVenn(tamoxifen,contrast=1,method=DBA_ALL_BLOCK,bAll=FALSE,bGain=TRUE,bLoss=FALSE)
dba.plotVenn(tamoxifen,contrast=1,method=DBA_ALL_BLOCK,bAll=FALSE,bGain=FALSE,bLoss=TRUE)