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methyAnalysis (version 1.14.0)

estimateMethySeq: Estimate the methylation level (Beta-value) of Methyl-Seq data

Description

Estimate the methylation level (Beta-value) of Methyl-Seq data, which is a VRanges object output by NGS pipeline

Usage

estimateMethySeq(seqVariant, coverage, CpGInfo = NULL, mergeStrand = TRUE, cleanVariant = TRUE, minCoverage = 10)

Arguments

seqVariant
a VRanges object output by NGS pipeline implemented in HTSeqGenie package
coverage
the genome coverage (a RleList object) output by NGS pipeline
CpGInfo
the precalculated CpG-site information (by identifyCpG function)
mergeStrand
whether to merge the AT and GA conversion on opposite strands
cleanVariant
whether to filter those non-CpG with full CT and GA conversion, or non CT and GA variations
minCoverage
minimum coverage for the variants

Value

a GRanges object with the Beta column shows the methylation levels

Details

See Also

Examples

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