polyester (version 1.8.3)

fpkm_to_counts: Turn FPKMs from a ballgown object into estimated counts for transcripts

Description

Turn FPKMs from a ballgown object into estimated counts for transcripts

Usage

fpkm_to_counts(bg, mean_rps = 1e+08, threshold = 0)

Arguments

bg

ballgown object created from real RNA-seq dataset

mean_rps

This should be the number of reads per sample in total for use in backing out the FPKM calculations

threshold

only estimate parameters from transcripts with mean FPKM measurements larger than threshold

Value

A matrix of counts with the same number of rows and columns as the ballgown object

Examples

library(ballgown)
  data(bg)
  countmat = fpkm_to_counts(bg, mean_rps=400000)