Learn R Programming

⚠️There's a newer version (1.16.0) of this package.Take me there.

Signac

Signac is a comprehensive R package for the analysis of single-cell chromatin data.

Documentation can be found at https://satijalab.org/signac/

Install

# Install Bioconductor
if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install()
setRepositories(ind=1:2)

# To install the current release
install.packages("Signac")

# To install the development version
install.packages("devtools")
devtools::install_github("timoast/signac", ref = "develop")

Copy Link

Version

Install

install.packages('Signac')

Monthly Downloads

7,177

Version

1.3.0

License

MIT + file LICENSE

Issues

Pull Requests

Stars

Forks

Maintainer

Tim Stuart

Last Published

July 12th, 2021

Functions in Signac (1.3.0)

BinarizeCounts

Binarize counts
AccessiblePeaks

Accessible peaks
AnnotationPlot

Plot gene annotations
AlleleFreq

Compute allele frequencies per cell
AddMotifs

Add DNA sequence motif information
Annotation

Annotation
BigwigTrack

Plot data from BigWig
AddChromatinModule

Add chromatin module
AverageCounts

Average Counts
AggregateTiles

Quantify aggregated genome tiles
CallPeaks

Call peaks
DepthCor

Plot sequencing depth correlation
CombineTracks

Combine genome region plots
ClusterClonotypes

Find relationships between clonotypes
CoveragePlot

Plot Tn5 insertion frequency over a region
Cells<-

Set and get cell barcode information for a Fragment object
CoverageBrowser

Genome browser
ClosestFeature

Closest Feature
CountsInRegion

Counts in region
ChromatinAssay-class

The ChromatinAssay class
CountFragments

Count fragments
FeatureMatrix

Feature Matrix
FRiP

Calculate fraction of reads in peaks per cell
DownsampleFeatures

Downsample Features
FindMotifs

FindMotifs
FindTopFeatures

Find most frequently observed features
Footprint

Transcription factor footprinting analysis
Cells.Fragment

ConnectionsToLinks

Cicero connections to links
CreateMotifMatrix

Create motif matrix
ConvertMotifID

Convert between motif name and motif ID
CellsPerGroup

Cells per group
GetLinkedPeaks

Get peaks linked to genes
GetMotifData

Retrieve a motif matrix
GeneActivity

Create gene activity matrix
CreateChromatinAssay

Create ChromatinAssay object
CreateFragmentObject

Create a Fragment object
GenomeBinMatrix

Genome bin matrix
CreateMotifObject

Create motif object
GetFragmentData

Get Fragment object data
FractionCountsInRegion

Fraction of counts in a genomic region
GetGRangesFromEnsDb

Extract genomic ranges from EnsDb object
Extend

Extend
ExpressionPlot

Plot gene expression
Fragment-class

The Fragment class
FragmentHistogram

Plot fragment length histogram
SubsetMatrix

Subset matrix rows and columns
GetCellsInRegion

Get cells in a region
GetFootprintData

Get footprinting data
Motif-class

The Motif class
MotifPlot

Plot DNA sequence motif
ReadMGATK

Read MGATK output
InsertionBias

Compute Tn5 insertion bias
PeakPlot

Plot peaks in a genomic region
GetTSSPositions

Find transcriptional start sites
IdentifyVariants

Identify mitochondrial variants
IntersectMatrix

Intersect genomic coordinates with matrix rows
TSSEnrichment

Compute TSS enrichment score per cell
GetIntersectingFeatures

Find intersecting regions between two objects
GetLinkedGenes

Get genes linked to peaks
Motifs

Get or set a motif information
RegionStats

Compute base composition information for genomic ranges
ValidateHash

Validate hashes for Fragment object
PlotFootprint

Plot motif footprinting results
MatchRegionStats

Match DNA sequence characteristics
LookupGeneCoords

Get gene coordinates
ValidateCells

Validate cells present in fragment file
UnifyPeaks

Unify genomic ranges
coverage,ChromatinAssay-method

Coverage of a ChromatinAssay object
ValidateFragments

Validate Fragment object
findOverlaps-methods

Find overlapping ranges for ChromatinAssay objects
FilterCells

Filter cells from fragment file
SplitFragments

Split fragment file by cell identities
RunTFIDF

Compute the term-frequency inverse-document-frequency
FindClonotypes

Find clonotypes
SetMotifData

Set motif data
NucleosomeSignal

NucleosomeSignal
Fragments

Get the Fragment objects
VariantPlot

Plot strand concordance vs. VMR
blacklist_dm6

Genomic blacklist regions for Drosophila dm6
StringToGRanges

String to GRanges
blacklist_dm3

Genomic blacklist regions for Drosophila dm3
UpdatePath

Update the file path for a Fragment object
TilePlot

Plot integration sites per cell
TSSPlot

Plot signal enrichment around TSSs
blacklist_hg38_unified

Unified genomic blacklist regions for Human GRCh38
Jaccard

Calculate the Jaccard index between two matrices
GRangesToString

GRanges to String
LinkPeaks

Link peaks to genes
blacklist_mm10

Genomic blacklist regions for Mouse mm10
LinkPlot

Plot linked genomic elements
Links

Get or set links information
reexports

Objects exported from other packages
RunChromVAR

Run chromVAR
blacklist_hg19

Genomic blacklist regions for Human hg19
RunSVD

Run singular value decomposition
seqinfo-methods

Access and modify sequence information for ChromatinAssay objects
blacklist_hg38

Genomic blacklist regions for Human GRCh38
as.ChromatinAssay

Convert objects to a ChromatinAssay
theme_browser

Genome browser theme
subset.Motif

Subset a Motif object
inter-range-methods

Inter-range transformations for ChromatinAssay objects
nearest-methods

Find the nearest range neighbors for ChromatinAssay objects
atac_small

A small example scATAC-seq dataset
blacklist_ce10

Genomic blacklist regions for C. elegans ce10
granges-methods

Access genomic ranges for ChromatinAssay objects
blacklist_ce11

Genomic blacklist regions for C. elegans ce11
head.Fragment

Return the first rows of a fragment file