canprot (version 1.1.0)

pdat_: Get Protein Expression Data

Description

Get differentially expressed proteins and amino acid compositions.

Usage

pdat_breast(dataset = 2020)
  pdat_colorectal(dataset = 2020)
  pdat_liver(dataset = 2020)
  pdat_lung(dataset = 2020)
  pdat_pancreatic(dataset = 2020)
  pdat_prostate(dataset = 2020)
  pdat_hypoxia(dataset = 2020)
  pdat_secreted(dataset = 2020)
  pdat_3D(dataset = 2020)
  pdat_glucose(dataset = 2020)
  pdat_osmotic_bact(dataset = 2020)
  pdat_osmotic_euk(dataset = 2020)
  pdat_osmotic_halo(dataset = 2020)
  .pdat_multi(dataset = 2020)
  .pdat_osmotic(dataset = 2017)

Arguments

dataset

character, dataset name

Value

A list consisting of:

dataset

Name of the dataset

description

Descriptive text for the dataset, used for making the tables in the vignettes (see mkvig)

pcomp

UniProt IDs together with amino acid compositions obtained using protcomp

up2

Logical vector with length equal to the number of proteins; TRUE for up-regulated proteins and FALSE for down-regulated proteins

Details

The pdat_ functions assemble lists of up- and down-regulated proteins and retrieve their amino acid compositions using protcomp. The result can be used with get_comptab to make a table of compositional metrics that can then be plotted with diffplot.

If dataset is 2020 (the default) or 2017, the function returns the names of all datasets in the compilation for the respective year.

Each dataset name starts with a reference key indicating the study (i.e. paper or other publication) where the data were reported. The reference keys are made by combining the first characters of the authors' family names with the 2-digit year of publication.

If a study has more than one dataset, the reference key is followed by an underscore and an identifier for the particular dataset. This identifier is saved in the variable named stage in the functions, but can be any descriptive text.

To retrieve the data, provide a single dataset name in the dataset argument. Protein expression data is read from the CSV files stored in extdata/expression/, under the subdirectory corresponding to the name of the pdat_ function. Some of the functions also read amino acid compositions (e.g. for non-human proteins) from the files in extdata/aa/.

Descriptions for each function:

• pdat_colorectal, pdat_pancreatic, pdat_breast, pdat_lung, pdat_prostate, and pdat_liver retrieve data for protein expression in different cancer types.

• pdat_hypoxia gets data for cellular extracts in hypoxia and pdat_secreted gets data for secreted proteins (e.g. exosomes) in hypoxia.

• pdat_3D retrieves data for 3D (e.g. tumor spheroids and aggregates) compared to 2D (monolayer) cell culture.

• .pdat_osmotic retrieves data for hyperosmotic stress, for the 2017 compilation only. In 2020, this compilation was expanded and split into pdat_osmotic_bact (bacteria), pdat_osmotic_euk (eukaryotic cells) and pdat_osmotic_halo (halophilic bacteria and archaea).

• pdat_glucose gets data for high-glucose experiments in eukaryotic cells.

• .pdat_multi retrieves data for studies that have multiple types of datasets (e.g. both cellular and secreted proteins in hypoxia), and is used internally by the specific functions (e.g. pdat_hypoxia and pdat_secreted).

Examples

# NOT RUN {
# List datasets in the 2017 complilation for colorectal cancer
pdat_colorectal(2017)
# Get proteins and amino acid compositions for one dataset
pdat_colorectal("JKMF10")
# }