plot.der

plot,der-method

plot,der,ANY-method


is a vector specifying what should be plotted. <code>1</code> means raw data, 
<code>2</code> means first derivative and <code>3</code> means second derivative. Any 
combination of mentioned values is valid, for example: c(1,2), c(1, 2, 3), 
c(2, 3). See Details and Examples.


what


is a <code>"logical"</code> argument. If <code>TRUE</code>, plot is added to existing 
plot.



is a <code>"logical"</code> argument. If <code>TRUE</code>, legend is added to the 
plot.


legend


is a vectors of colors used in plot. Must have length 3 for the original function, first, 
and second derivative, respectively.


plot.colors


Arguments to be passed further to the plot function, such as graphical 
parameters.


hplot

A collection of functions to pre-process amplification curve data from polymerase chain reaction (PCR) or isothermal amplification reactions. Contains functions to normalize and baseline amplification curves, to detect both the start and end of an amplification reaction, several smoothers (e.g., LOWESS, moving average, cubic splines, Savitzky-Golay), a function to detect false positive amplification reactions and a function to determine the amplification efficiency. Quantification point (Cq) methods include the first (FDM) and second approximate derivative maximum (SDM) methods (calculated by a 5-point-stencil) and the cycle threshold method. Data sets of experimental nucleic acid amplification systems (VideoScan HCU, capillary convective PCR (ccPCR)) and commercial systems are included. Amplification curves were generated by helicase dependent amplification (HDA), ccPCR or PCR. As detection system intercalating dyes (EvaGreen, SYBR Green) and hydrolysis probes (TaqMan) were used.

plot.der: Plot `der` objects

Description

Usage

Arguments

See Also

Examples