ABC.RAP (version 0.9.0)

process.ABC.RAP: An automated analysis applying all ABC.RAP functions in one script

Description

This function processes the ABC.RAP workflow automatically

Usage

process.ABC.RAP(x, cases_column_1, cases_column_n, controls_column_1, controls_column_n, ttest_cutoff, meth_cutoff, unmeth_cutoff, high_meth, low_meth)

Arguments

x

The normalised beta values in a data matrix format, where conditions are arranged in columns and cg probes are arranged in rows.

cases_column_1

The first column (column number) for cases in the filtered dataset

cases_column_n

The last column (column number) for cases in the filtered dataset

controls_column_1

The first column (column number) for controls in the filtered dataset

controls_column_n

The last column (column number) for controls in the filtered dataset

ttest_cutoff

The cutoff level to filter insignificant p-values

meth_cutoff

The cutoff level for the methylation difference between cases and controls (cases minus controls)

unmeth_cutoff

The cutoff level for the methylation difference between controls and cases (controls minus cases). Consequently, it requires a negative value.

high_meth

The upper margin for the highly methylated probes

low_meth

The lower margin for the low methylation

Examples

data(test_data)
data(nonspecific_probes)
data(annotation_file)
process.ABC.RAP(test_data, 1, 2, 3, 4, 1e-3,  0.5, -0.5, 0.94, 0.06)