targets.micro: Example of target file
Description
Example of target file
Format
A data frame with 4 observations on the following 5 variables.
FileName- names of the Files
Ast.txt Bst.txt Aunst.txt Bunst.txt Treatment- Assigns level for Treatment Effect to each File (mandatory)
GErep- a numeric vector tha numerates the FACTOR of the Treatment Effect (mandatory)
Subject- Assigns level for Subject Effect to each File
Details
It is a tab-delimited text format file.
The target file is created by the user with the intention of
carrying out a differential expression analysis
in future steps using 'limma'. Here is where the
factors that are going to be included in the
linear model that is fitted to each gen are specified.
The targets file assigns each data file to a particular
experimental conditions.
First column 'FileName' is mandatory and includes the
image data files names. Second column
'Treatment' is also mandatory and includes the treament
effect. Third column 'GErep' is also mandatory, and
includes the treatment effect in a numeric code, from
1 to n, being n the number of Treatment effect levels.
References
Gordon K. Smyth, M. Ritchie, N. Thorne, J. Wettenhall (2007). limma:
Linear Models for Microarray Data User's Guide.