log2CPM

qcounts

lib.size


Compute log2(counts per mil reads) and library size for each sample


Sequencing and microarray samples often are collected or
processed in multiple batches or at different times. This often
produces technical biases that can lead to incorrect results in
the downstream analysis. BatchQC is a software tool that
streamlines batch preprocessing and evaluation by providing
interactive diagnostics, visualizations, and statistical
analyses to explore the extent to which batch variation impacts
the data. BatchQC diagnostics help determine whether batch
adjustment needs to be done, and how correction should be
applied before proceeding with a downstream analysis. Moreover,
BatchQC interactively applies multiple common batch effect
approaches to the data, and the user can quickly see the
benefits of each method. BatchQC is developed as a Shiny App.
The output is organized into multiple tabs, and each tab
features an important part of the batch effect analysis and
visualization of the data. The BatchQC interface has the
following analysis groups: Summary, Differential Expression,
Median Correlations, Heatmaps, Circular Dendrogram, PCA
Analysis, Shape, ComBat and SVA.

log2CPM: Compute log2(counts per mil reads) and library size for each sample

Description

Usage

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Examples