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CODEX (version 1.4.0)

A Normalization and Copy Number Variation Detection Method for Whole Exome Sequencing

Description

A normalization and copy number variation calling procedure for whole exome DNA sequencing data. CODEX relies on the availability of multiple samples processed using the same sequencing pipeline for normalization, and does not require matched controls. The normalization model in CODEX includes terms that specifically remove biases due to GC content, exon length and targeting and amplification efficiency, and latent systemic artifacts. CODEX also includes a Poisson likelihood-based recursive segmentation procedure that explicitly models the count-based exome sequencing data.

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Version

Version

1.4.0

License

GPL-2

Maintainer

Yuchao Jiang

Last Published

February 15th, 2017

Functions in CODEX (1.4.0)

CODEX-package

A Normalization and Copy Number Variation Detection Method for Whole Exome Sequencing
getbambed

Get bam file directories, sample names, and exonic positions
getcoverage

Get depth of coverage from whole exome sequencing
segment

Recursive segmentation algorithm for CNV detection and genotyping
getmapp

Get mappability for each exonic target
mappDemo

Demo data pre-stored for mappability.
mapp_ref

Position reference for pre-computed mappability results.
coverageObjDemo

Demo data pre-stored for coverageObj.
bambedObjDemo

Demo data pre-stored for bambedObj.
getgc

Get GC content for each exonic target
qc

Quality control procedure for depth of coverage
normalize

Normalization of read depth from whole exome sequencing
normalize2

Normalization of read depth from whole exome sequencing under the case-control setting
qcObjDemo

Demo data pre-stored for qcObj.
mappability

Pre-computed mappabilities
normObjDemo

Demo data pre-stored for normObj.
gcDemo

Demo data pre-stored for GC content.
choiceofK

Determine the number of latent factors K.