Usage
adjustFluo(data, transformation, BGmethod, maxMix, reference, prior.pi, flex.reps, flexmethod, image.type, savePlot, seed, dateIndex)
Arguments
data
List. A list with the fluorescence signal information of both channels.
BGmethod
Character string. The type of image background correction to be performed.
One of "normexp" or "subtract".
maxMix
Integer. The maximum number of components to fit into the mixture of
regressions model. If maxMix=1 or if the the optimal number of the estimated components
is 1, the model reduces to the classical 2-way ANOVA.
reference
Numeric vector. Specifies the runs to be used as baseline (iteratively).
prior.pi
Float. The prior probability to accept a component.
flex.reps
Integer. The iterations of the Expectation-Maximization algorithm to estimate
the flexmix model.
flexmethod
Character string. A method to estimate the optimal number of flexmix
components. One of "BIC", "AIC", "ICL".
image.type
Character string. A triplet of IDs to characterize the type of images under study.
savePlot
Character string. The directory to store the plots or an option to print them on the screen.
seed
Integer. An optional seed number for the Random Number Generator.
dateIndex
Character string. a date index to be used in saving the output files.
transform
Character string. The type of transformation to be performed. One
of "bc" (Box-Cox), "log", "log10" or "asinh".