CONFESS (version 1.0.2)

getCoordinates_stats: getCoordinates_stats

Description

It finds the spot coordinates using the spot centers or BF image modeling

Usage

getCoordinates_stats(centerR, centerG, minDiff, chaImgs, ll, ws, estCenter)

Arguments

centerR

Data matrix. The location statistics in one channel.

centerG

Data matrix. The location statistics in thew other channel.

minDiff

Float. the mu_hat of the H0: image-to-noise ratio = log(foreground_signal) - log(background_signal) = mu_hat. Rejection of H0 implies that the identified spot is brighter than background. Default is 0.5.

chaImgs

List. A list of the channel images (data matrices) of a sample.

ll

Data matrix. An internal parameter specifying the spot center.

ws

List. An internal parameter specifying the spot center.

estCenter

Data matrix. The estimated spot center by BF image modelling.

Value

A series of location estimates includying the channel-specific spot center and spot areas