CONFESS (version 1.0.2)

joinAreas: joinAreas

Description

It estimates the spot area by joining the red and green bright spot location estimates.

Usage

joinAreas(areaR, areaG, center, chaImg, areaBased, warning)

Arguments

areaR

Data matrix. The bright (spot) coordinates in one channel.

areaG

Data matrix. The bright (spot) coordinates in the other channel.

center

Data matrix. The 2-dimensional location of the spot's center.

areaBased

Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated.

warning

Character string. An indicator of the estimation type that has been internally performed, i.e. fluorescence-based or BF image modelling.

chaImgs

Data matrix. The channel binary segmented image data that are used for reference to obtain the area of interest.

Value

The coordinates of the spot area and its length in pixels.