CONFESS (version 1.0.2)

measureB: measureB

Description

It estimates the background signal for an image.

Usage

measureB(img, area, iter, BFarea)

Arguments

img

Data matrix. The matrix of image data from a channel.

area

Data matrix. The bright (spot) coordinates around the spot center.

iter

Integer. A number of iterations (pseudo-spots) to be summarized.

BFarea

Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated.

Value

The background image estimates