It estimates a series of spot statistics on each channel.
Usage
spotCenter(img, foregroundCut, howbig, ImgLimits)
Arguments
img
Data matrix. The channel image data.
foregroundCut
Numeric vector. The binary segmentation image analysis cutoffs
for normalized image data. Pixels with normalized signals higher than the cutoff
belong to foreground.
howbig
Integer. An user defined value of the minimum expected spot size.
ImgLimits
a cutoff that determines where the spot is supposed to be found.