CONFESS (version 1.0.2)

straightColLines: straightColLines

Description

It identifies the vertical BF image characteristic lines.

Usage

straightColLines(img, pattern.search, cut = seq(0.3, 1.5, 0.02), ImgLimits, chip.type)

Arguments

img

Data matrix. The BF image data matrix.

pattern.search

Integer. A cutoff to find horizontal and vertical lines on the chip.

cut

Integer. A normalized signal cutoff above which a pixel is considered as foreground.

ImgLimits

Integer. It instructs the algorithm to find spots in a certain central image area. For example, for a 512 x 512 image with cutSides = 50, it will search for spots in the central area [ImgLimits:(512-ImgLimits),ImgLimits:(512-ImgLimits)] of the image matrix.

chip.type

Character string. It specifies the type of Fluidigm chip to be analyzed.

Value

Estimated vertical BF characteristic lines.