createUniqueCellNames

Create unique cell names from sample IDs and cell IDs

internal

Sample and cell filtering as well as visualisation of output metrics from 'Cell Ranger' by Grace X.Y. Zheng et al. (2017) <doi:10.1038/ncomms14049>. 'CRMetrics' allows for easy plotting of output metrics across multiple samples as well as comparative plots including statistical assessments of these. 'CRMetrics' allows for easy removal of ambient RNA using 'SoupX' by Matthew D Young and Sam Behjati (2020) <doi:10.1093/gigascience/giaa151> or 'CellBender' by Stephen J Fleming et al. (2022) <doi:10.1101/791699>. Furthermore, it is possible to preprocess data using 'Pagoda2' by Nikolas Barkas et al. (2021) <https://github.com/kharchenkolab/pagoda2> or 'Seurat' by Yuhan Hao et al. (2021) <doi:10.1016/j.cell.2021.04.048> followed by embedding of cells using 'Conos' by Nikolas Barkas et al. (2019) <doi:10.1038/s41592-019-0466-z>. Finally, doublets can be detected using 'scrublet' by Samuel L. Wolock et al. (2019) <doi:10.1016/j.cels.2018.11.005> or 'DoubletDetection' by Gayoso et al. (2020) <doi:10.5281/zenodo.2678041>. In the end, cells are filtered based on user input for use in downstream applications.

Rasmus Rydbirk

CRMetrics

Cell Ranger Output Filtering and Metrics Visualization

Fabienne Kick

Henrietta Holze

Xian Xin

createUniqueCellNames function

<dl><dt>cms</dt>
<dd>list List of count matrices, should be named (optional)</dd>
<dt>samples</dt>
<dd>character Optional, list of sample names</dd>
<dt>sep</dt>
<dd>character Separator between sample IDs and cell IDs (default = "!!")</dd></dl>

Arguments

Create unique cell names — createUniqueCellNames

<dl>

<dt>cms</dt>
<dd>list List of count matrices, should be named (optional)</dd>


<dt>samples</dt>
<dd>character Optional, list of sample names</dd>


<dt>sep</dt>
<dd>character Separator between sample IDs and cell IDs (default = "!!")</dd>

</dl>

createUniqueCellNames: Create unique cell names

Description

Usage

Arguments