percFilter

Calculate percentage of filtered cells based on the filter

internal

Sample and cell filtering as well as visualisation of output metrics from 'Cell Ranger' by Grace X.Y. Zheng et al. (2017) <doi:10.1038/ncomms14049>. 'CRMetrics' allows for easy plotting of output metrics across multiple samples as well as comparative plots including statistical assessments of these. 'CRMetrics' allows for easy removal of ambient RNA using 'SoupX' by Matthew D Young and Sam Behjati (2020) <doi:10.1093/gigascience/giaa151> or 'CellBender' by Stephen J Fleming et al. (2022) <doi:10.1101/791699>. Furthermore, it is possible to preprocess data using 'Pagoda2' by Nikolas Barkas et al. (2021) <https://github.com/kharchenkolab/pagoda2> or 'Seurat' by Yuhan Hao et al. (2021) <doi:10.1016/j.cell.2021.04.048> followed by embedding of cells using 'Conos' by Nikolas Barkas et al. (2019) <doi:10.1038/s41592-019-0466-z>. Finally, doublets can be detected using 'scrublet' by Samuel L. Wolock et al. (2019) <doi:10.1016/j.cels.2018.11.005> or 'DoubletDetection' by Gayoso et al. (2020) <doi:10.5281/zenodo.2678041>. In the end, cells are filtered based on user input for use in downstream applications.

Rasmus Rydbirk

CRMetrics

Cell Ranger Output Filtering and Metrics Visualization

Fabienne Kick

Henrietta Holze

Xian Xin

percFilter function

<dl><dt>filter.data</dt>
<dd>Data frame containing the mitochondrial fraction, depth and doublets per sample.</dd>
<dt>filter</dt>
<dd>The variable to filter (default = "mito")</dd>
<dt>no.vars</dt>
<dd>numeric Number of variables (default = 1)</dd></dl>

Arguments

Calculate percentage of filtered cells — percFilter

<dl>

<dt>filter.data</dt>
<dd>Data frame containing the mitochondrial fraction, depth and doublets per sample.</dd>


<dt>filter</dt>
<dd>The variable to filter (default = "mito")</dd>


<dt>no.vars</dt>
<dd>numeric Number of variables (default = 1)</dd>

</dl>

percFilter: Calculate percentage of filtered cells

Description

Usage

Value

Arguments