ChIPseqR (version 1.26.0)

ChIPseqR-package: Identifying Protein Binding Sites in High-Throughput Sequencing Data

Description

ChIPseqR provides a set of functions for the analysis of ChIP-seq data. Protein binding sites are located by identifying a characteristic pattern of peaks in read counts on both DNA strands.

Details

Package:

ChIPseqR

Type:

Package

Version:

1.13.1

Date:

2012-12-11

License:

GPL (>=2)

LazyLoad:

yes

The easiest way to obtain binding site predictions for nucleosomes is to use simpleNucCall. This provides a simple interface to callBindingSites. This function operates on AlignedRead objects and provides useful defaults for nucleosome analysis. Parameters can be adjusted to detect the presence of other DNA binding proteins, e.g. transcription factors. If more fine control is desired the following steps will produce binding site predictions:

strandPileup:

Turn mapped reads into read counts along the genome.

startScore:

Score potential binding sites.

getCutoff:

Determine cutoff required to achieve desired false discovery rate.

pickPeak:

Find all peaks in the binding site score that exceed the significance threshold determined by getCutoff. These are the predicted binding sites.

References

Humburg, P., Helliwell, C., Bulger, D. & Stone, G. ChIPseqR: Analysis of ChIP-seq Experiments. BMC Bioinformatics 12, 39+ (2011).

See Also

ShortRead

Examples

	## See 'simpleNucCall' for examples of how to obtain nucleosome predictions.