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CleanBSequences (version 2.3.0)

DNAStringSetTPR: Curing of biological sequences

Description

Curates biological sequences of two restriction enzyme primers or cloning vectors.This cleaning is required for techniques such as cDNA-AFLP.This cleaning is required for techniques such as cDNA-AFLP.

Usage

DNAStringSetTPR(SEQs, PrimerF, PrimerR)

Value

clean biological sequences and visualization of the alignments

Arguments

SEQs

file with fasta format containing biological sequences that are to be cleaned.

PrimerF

dnastring containing the foward primer/vector sequences to be removed.

PrimerR

dnastring containing the reverse primer/vector sequences to be removed.

Author

Florencia I Pozzi, Silvina A. Felitti

Examples

Run this code
SEQs = readDNAStringSet(system.file("sequences","SeqInputTPR.fasta", package = "CleanBSequences"))
PrimerR= DNAString ("GACTGCGTACCATGC")
PrimerF = DNAString("GATGAGTCCTGACCGAA")
DNAStringSetTPR (SEQs,PrimerF,PrimerR)

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