rmMultiPCRChimera

rmMultiPCRChimera,character,Hits,integer-method

A set of read names, used for identifying chimeric read sets

readnames

A mapping between indices of reads and a set of pcr primers

pcrhits

location of chimeric reads within the bam

chimera_idxs

Display information about the chimeras (Default: TRUE)

verbose


Finds and removes sets of chimeric read alignments
that overlap more than one guide, i.e. that cannot be unambiguously
assigned to a single guide.


CrispRVariants provides tools for analysing the results of a
CRISPR-Cas9 mutagenesis sequencing experiment, or other sequencing experiments
where variants within a given region are of interest. These tools allow users to
localize variant allele combinations with respect to any genomic location (e.g.
the Cas9 cut site), plot allele combinations and calculate mutation rates with
flexible filtering of unrelated variants.

rmMultiPCRChimera: Remove chimeric reads overlapping multiple primers

Description

Usage

Arguments

Value