A data frame with 10 rows and 20 columns, the columns represents samples, say first 10 columns 1 to 10 being control and 11 to 20 being experiment. Whereas, the rows of the dataset contains genes. First 5 out of 10 genes, gene1-gene5 are the true DEGs as the expression values for the first 10 samples are ~13 times higher than the rest.