EZbakR (version 0.1.0)

create_fraction_design: Generate a fraction_design table for EstimateFractions.

Description

A fraction_design table denotes what populations of labeled/unlabeled RNA are present in your data. A fraction_design table as one column for each mutation type (e.g., TC) present in your cB file, and one column named "present". Each entry is either TRUE or FALSE. The rows include all possible combinations of TRUE and FALSE for all mutation types columns. A value of TRUE in a mutation type column represents a population of reads that have high amounts (on average) of that mutation type. For example, if your fraction_design table has mutation type columns "TC" and "GA", the row with TC == TRUE and GA == FALSE represents a population of reads with high T-to-C mutation content and low G-to-A mutation content. In other words, these are reads from RNA synthesized in the presence of s4U but not s6G. If such a population exists in your data, the "present" column for that row should have a value of TRUE.

Usage

create_fraction_design(mutrate_populations)

Value

A fraction_design table that assumes that every possible combination of mutational populations listed in mutrate_populations are present in your data. The present column can be modified if this assumption is incorrect. This default is chosen as it will in theory work for all analyses, it may just be unnecessarily inefficient and estimate the abundance of populations that don't exist.

Arguments

mutrate_populations

Character vector of the set of mutational populations present in your data. For example, s4U fed data with standard nucleotide recoding chemistry (e.g., TimeLapse, SLAM, TUC, AMUC, etc.) would have a mutrate_populations of c("TC"). Dual labeling experiments with s4U and s6G feeds would have a mutrate_populations of c("TC", "GA").

Examples

# Standard, single-label NR-seq
fd <- create_fraction_design(c("TC"))

# Dual-label NR-seq
fd2 <- create_fraction_design(c("TC", "GA"))

# Adjust dual-label output for TILAC
fd2$present <- ifelse(fd2$TC & fd2$GA, FALSE, fd2$present)