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Fragment Analysis And Capillary Sequencing Tool Kit

Functions to import Applied Biosystems data files and process them in a highly flexible and interfaced framework. RT-MLPA application (gene-expression profiling and classification) is described in Mareschal, Ruminy et al (2015) doi:10.1016/j.jmoldx.2015.01.007. Gene-fusion detection is described in Mareschal, Palau et al (under review). Examples are provided for genotyping applications as well.

Availability

A short presentation of the project can be found on my personnal website.

Release sources and builds can be found in the CRAN repository :

Development sources are freely available on GitHub :

All this material is freely distributed under the GNU Public License 3, shipped with sources and builds.

Support

Bugs, suggestions and help requests should be submitted using GitHub's Issues (see 'Availability').

Changelog

Release updates are tracked by the NEWS.Rd file, browsable on the CRAN repository (see 'Availability').

Individual commits can be followed on GitHub (see 'Availability').

Credits

Sylvain Mareschal <mareschal@ovsa.fr>

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Version

Install

install.packages('FSAtools')

Monthly Downloads

31

Version

2.0.5

License

GPL (>= 3)

Maintainer

Sylvain Mareschal

Last Published

August 18th, 2023

Functions in FSAtools (2.0.5)

designFile

Process interface's design file
classify

Apply the binary predictor to FSA peaks
plot.fsa

Plot method for "fsa" objects
multiplot

Wrapper to layout
read.fsa

Imports a .fsa file from Applied Biosystems
print.fsa

Print method for "fsa" objects
train

Training function for binary predictors
plot.fsaModel

Plot method for "fsaModel" objects
peaks.fsa

Get maximal value in ranges
wav2RGB

Converts light wavelengths to RGB colors
read.sanger

Imports a .ab1 file from Applied Biosystems corresponding to Sanger sequencing
read.abif

Read ABIF formatted files
fusions.process

LD-RTPCR fusion identification by Sanger
export.attr

Print an attribute of a 'fsa' object to a file
align.fsa

Aligns peaks using size ladder
genotype.fsa

Calls alleles for a SNP genotyping experiment
generic.log

Wrapper for generic.process
add.model

Object constructor for binary predictors
filter.fsa

Applies filter() to a "fsa" object
generic.process

Processing multiple FSA files