FracFixR-package

A compositional statistical framework for absolute proportion estimation 
between fractions in RNA sequencing data. FracFixR addresses the fundamental
challenge in fractionated RNA-seq experiments where library preparation and
sequencing depth obscure the original proportions of RNA fractions.

internal

A compositional statistical framework for absolute proportion
estimation between fractions in RNA sequencing data. 'FracFixR' addresses
the fundamental challenge in fractionated RNA-seq experiments where library
preparation and sequencing depth obscure the original proportions of RNA
fractions. It reconstructs original fraction proportions using non-negative
linear regression, estimates the "lost" unrecoverable fraction, corrects
individual transcript frequencies, and performs differential proportion
testing between conditions. Supports any RNA fractionation protocol including
polysome profiling, sub-cellular localization, and RNA-protein complex isolation.

Alice Cleynen

FracFixR

Compositional Statistical Framework for RNA Fractionation
Analysis

Agin Ravindran

Nikolay Shirokikh

FracFixR-package function

Maintainer: Alice Cleynen <a href="/link/alice.cleynen%40cnrs.fr?package=FracFixR&version=1.0.0" data-mini-rdoc="FracFixR::alice.cleynen@cnrs.fr">alice.cleynen@cnrs.fr</a> (<a href="https://orcid.org/0000-0001-8083-0204">ORCID</a>)
Authors:<ul>
<li>Agin Ravindran</li>
<li>Nikolay Shirokikh <a href="/link/nikolay.shirokikh%40uwa.edu.au?package=FracFixR&version=1.0.0" data-mini-rdoc="FracFixR::nikolay.shirokikh@uwa.edu.au">nikolay.shirokikh@uwa.edu.au</a> (<a href="https://orcid.org/0000-0001-8249-358X">ORCID</a>)</li>
</ul>

Author

FracFixR: Fraction Correction Framework for RNA-seq Data — FracFixR-package

Maintainer: Alice Cleynen <a href='mailto:alice.cleynen@cnrs.fr'>alice.cleynen@cnrs.fr</a> (<a href='https://orcid.org/0000-0001-8083-0204'>ORCID</a>)
Authors:<ul>
<li>Agin Ravindran</li>
<li>Nikolay Shirokikh <a href='mailto:nikolay.shirokikh@uwa.edu.au'>nikolay.shirokikh@uwa.edu.au</a> (<a href='https://orcid.org/0000-0001-8249-358X'>ORCID</a>)</li>
</ul>

FracFixR-package: FracFixR: Fraction Correction Framework for RNA-seq Data

Description

Arguments

Author