swap_channel

Swaps Channels within `IFC_data` object

internal

Contains several tools to treat imaging flow cytometry data from 'ImageStream®' and 'FlowSight®' cytometers ('Amnis®' 'Cytek®'). Provides an easy and simple way to read and write .fcs, .rif, .cif and .daf files. Information such as masks, features, regions and populations set within these files can be retrieved for each single cell. In addition, raw data such as images stored can also be accessed. Users, may hopefully increase their productivity thanks to dedicated functions to extract, visualize, manipulate and export 'IFC' data. Toy data example can be installed through the 'IFCdata' package of approximately 32 MB, which is available in a 'drat' repository <https://gitdemont.github.io/IFCdata/>. See file 'COPYRIGHTS' and file 'AUTHORS' for a list of copyright holders and authors.

Yohann Demont

Tools for Imaging Flow Cytometry

Gautier Stoll

Guido Kroemer

Jean-Pierre Marolleau

Loïc Garçon

swap_channel function

<dl><dt>obj</dt>
<dd>an `IFC_data` object extracted with features extracted.</dd>
<dt>chan1, chan2</dt>
<dd>an integer index of channel. 'chan1' and 'chan2' should be different.</dd>
<dt>BF</dt>
<dd>should 'from' channel be considered as brightfield. Default is TRUE.</dd>
<dt>MODE</dt>
<dd>collection mode (as retrieved by getInfo) determining the range. Default is 1.</dd></dl>

Arguments

Channel Swap — swap_channel

<dl>

<dt>obj</dt>
<dd>an `IFC_data` object extracted with features extracted.</dd>


<dt>chan1, chan2</dt>
<dd>an integer index of channel. 'chan1' and 'chan2' should be different.</dd>


<dt>BF</dt>
<dd>should 'from' channel be considered as brightfield. Default is TRUE.</dd>


<dt>MODE</dt>
<dd>collection mode (as retrieved by getInfo) determining the range. Default is 1.</dd>

</dl>

swap_channel: Channel Swap

Description

Usage

Value

Arguments

Details