toEllipse

Helper to transform gate boundaries to ellipsoid polygon.

internal

Contains several tools to treat imaging flow cytometry data from 'ImageStream®' and 'FlowSight®' cytometers ('Amnis®' 'Cytek®'). Provides an easy and simple way to read and write .fcs, .rif, .cif and .daf files. Information such as masks, features, regions and populations set within these files can be retrieved for each single cell. In addition, raw data such as images stored can also be accessed. Users, may hopefully increase their productivity thanks to dedicated functions to extract, visualize, manipulate and export 'IFC' data. Toy data example can be installed through the 'IFCdata' package of approximately 32 MB, which is available in a 'drat' repository <https://gitdemont.github.io/IFCdata/>. See file 'COPYRIGHTS' and file 'AUTHORS' for a list of copyright holders and authors.

Yohann Demont

Tools for Imaging Flow Cytometry

Gautier Stoll

Guido Kroemer

Jean-Pierre Marolleau

Loïc Garçon

toEllipse function

<dl><dt>gate</dt>
<dd>list containing x and y ellipse boundaries coordinates.</dd>
<dt>theta</dt>
<dd>rotation angle of the ellipse. Default is 2*pi. It should not be modified since ellipse gate are axis-aligned.</dd>
<dt>npoints</dt>
<dd>number of polygon vertices desired.</dd></dl>

Arguments

Ellipsoid Polygon Constructor — toEllipse

<dl>

<dt>gate</dt>
<dd>list containing x and y ellipse boundaries coordinates.</dd>


<dt>theta</dt>
<dd>rotation angle of the ellipse. Default is 2*pi. It should not be modified since ellipse gate are axis-aligned.</dd>


<dt>npoints</dt>
<dd>number of polygon vertices desired.</dd>

</dl>

toEllipse: Ellipsoid Polygon Constructor

Description

Usage

Arguments