This function analyzes raw abundance data from a Thermal Proteome Profiling experiment and calculates melt temperatures and melt shifts for each protein in the experiment.
InflectWorkflow(Rsq, NumSD, Temperature, Rep, SourcePath, OutputPath)the cutoff to be used for the melt shift curve fit. An example entry would be 0.95
the standard deviation cutoff to be used for the calculated melt shifts. For example, if NumSD = 2, proteins with melt shifts greater than 2 standard deviations from the mean will be considered significant.
the temperatures from the heat treatment procedure. An example entry Temperature<-c(25,35,39.3,50.1,55.2,60.7,74.9,90)
the number of replicate experiments to be analyzed
The path for the source data
The path for the output data
xlsx files with calculated melt shift for each protein in the experiment