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LorMe (version 2.0.1)

LorMe-package: LorMe: Lightweight One-Line Resolving Microbial Ecology Program

Description

Package for lightweight, one-line microbial ecology analysis including diversity, differential abundance, and network analysis.

Arguments

Data I/O and interoperability

  • tax_summary - Build S4 object from biom/meta files.

  • Trans_from_phylo - Convert phyloseq objects to LorMe.

  • Trans_to_phylo - Export LorMe to phyloseq.

  • Trans_from_microeco - Convert microtable (microeco) to LorMe.

  • Trans_to_microeco - Export LorMe to microtable.

  • sub_tax_summary - Subset LorMe object by metadata.

  • add_tree - Attach a phylogenetic tree to LorMe object.

Global configuration

  • LorMe_options - One-line option manager (analysis level, colours, network thresholds, etc.).

  • object_config - Interactive helper to set treatment column and comparison list.

One-line pipeline

  • LorMe_pipeline - Run alpha diversity, beta diversity, differential abundance, indicator species, per-treatment and combined networks in a single call; results returned as a tidy list.

Community profiling

  • Alpha_diversity_calculator - Alpha diversity analysis including Shannon, Simpson, Chao1, etc.

  • structure_plot - Community structure analysis including PCA/PCoA/NMDS.

  • community_plot - Community composition analysis including Bar, area and alluvial plots.

  • Top_taxa - Calculate most abundant taxon across samples.

Differential analysis

  • Deseq_analysis - DESeq analysis.

  • indicator_analysis - Indicator analysis.

  • differential_bar - Differential bar analysis.

  • volcano_plot / manhattan - Volcano and Manhattan visualizations.

Network analysis

  • network_analysis - Co-occurrence network (Cytoscape/Gephi ready).

  • network_withdiff - Meta-network integrating differential taxa.

  • network_visual / network_visual_re - Statistics and visualizations.

  • Module_composition / Module_abundance - Module summaries and per-sample abundances.

  • nc / NC_remove - Natural connectivity metrics.

Utility functions

  • Filter_function - Pre-filter OTU/ASV by prevalence or abundance.

  • theme_zg - ggplot2 theme optimized for figures.

  • color_scheme - Ten built-in color ramps.

  • auto_signif_test - Automatic multiple comparison.

  • compare_plot - Quick comparison plot generator.

Author

Ningqi Wang