threshold

a number between 0 to 100 denoting the percentage of allowable missing.  If the percentage of missing is greater than this value, the row (gene) is not retained.

missing.percent.thresh


<code>scr</code> screens the dataset in wide format according to Ct threshold and missing.percent.thresh (for each gene, the percentage of samples is missing).
sets all values above Ct threshold to NA and then removes all rows of genes with number of missing above missing.percent.thresh.


An adaptive normalization algorithm that selects housekeeping genes
based on the sample level variability in the data. This is suitable for any data
obtained from RT-qPCR assays.  A manuscript describing the method is submitted
to Genome Biology under ``MiRA-norm: An Adaptive Method for the Normalization of MicroRNA Array Data``, Yuda Zhu et al.

scr: Replacing values above ct to NA

Description

Usage

Arguments