MicroSEC (version 2.1.6)

fun_analysis: Analyzing function.

Description

This function analyzes the filtering results.

Usage

fun_analysis(
  msec,
  mut_depth,
  short_homology_search_length,
  min_homology_search,
  threshold_p,
  threshold_hairpin_ratio,
  threshold_short_length,
  threshold_distant_homology,
  threshold_soft_clip_ratio,
  threshold_low_quality_rate,
  homopolymer_length
)

Value

msec

Arguments

msec

Mutation filtering information.

mut_depth

Mutation coverage data.

short_homology_search_length

Small sequence for homology search.

min_homology_search

The sequence length for homology search.

threshold_p

The largest p value of significant errors.

threshold_hairpin_ratio

The smallest hairpin read ratio.

threshold_short_length

Reads shorter than that are analyzed.

threshold_distant_homology

The smallest rate of reads from other regions.

threshold_soft_clip_ratio

The rate of soft-clipped reads.

threshold_low_quality_rate

The smallest rate of low quality bases.

homopolymer_length

The smallest length of homopolymers.

Examples

data(msec_summarized)
data(mut_depth_checked)
fun_analysis(msec = msec_summarized,
             mut_depth = mut_depth_checked,
             short_homology_search_length = 4,
             min_homology_search = 40,
             threshold_p = 10 ^ (-6),
             threshold_hairpin_ratio = 0.50,
             threshold_short_length = 0.75,
             threshold_distant_homology = 0.15,
             threshold_soft_clip_ratio = 0.50,
             threshold_low_quality_rate = 0.1,
             homopolymer_length = 15
)