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NanoStringDiff (version 1.2.0)

estNormalizationFactors: estimate normalization factors,include positive size factors, background noise, housekeeping size factors.

Description

This function estimates positive size factors, background noise and housekeeping size factors for the input "NanoStringSet" object and return the same object with positiveFactor, negativeFactor and housekeepingFactor slots filled or replaced.

Usage

estNormalizationFactors(NanoStringData)

Arguments

NanoStringData
An object of "NanoStringSet" class.

Value

The same "NanoStringSet" object with positiveFactor, negativeFactor and housekeepingFactor field filled or replaced.

Examples

Run this code
data(NanoStringData)
NanoStringData=estNormalizationFactors(NanoStringData)
pf=positiveFactor(NanoStringData)
nf=negativeFactor(NanoStringData)
hf=housekeepingFactor(NanoStringData)

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