NanoStringDiff (version 1.2.0)

housekeepingControl: Acessor functions for the 'housekeepingControl' slot in a NanoStringSet object.

Description

user-defined housekeeping control genes can be used to estimate housekeeping factors to adjust variation caused by different sample input.

Usage

"housekeepingControl"(object)
"housekeepingControl"(object) <- value

Arguments

object

A NanoStringSet object.

value

A matrix with housekeeping control genes.

Value

Details

NanoString nCounter analyzer also contains probes for a set of species-specific mRNA housekeeping(reference) genes that are not spike-in the system. Nanostring recommends at least three housekeeping genes, but the more that are included, the more accurate the normalization will be. Housekeeping control genes are expected consistent in their expression levels.

See Also

housekeepingFactor

Examples

data(NanoStringData)
## obtain housekeeping control genes
housekeepingControl(NanoStringData)

## assign a matrix
n=ncol(exprs(NanoStringData))
r=nrow(housekeepingControl(NanoStringData))
housekeeping=matrix(rpois(r*n,1000),ncol=n)
housekeepingControl(NanoStringData)=housekeeping