NanoStringDiff (version 1.2.0)

positiveControl: Acessor functions for the 'positiveControl' slot in a NanoStringSet object.

Description

nCounter Analyzer has positive spike-in RNA hybridization controls for each sample which can be used to estimate the overall efficiency of hybridization and recovery for each sample.

Usage

"positiveControl"(object)
"positiveControl"(object) <- value

Arguments

object

A NanoStringSet object.

value

A matrix with six positive control genes.

Value

Details

Positive control genes are provided by NanoString nCounter technology. For each sample, nCounter provide six positive controls corresponding to six different concentrations in the 30 ul hybridzation: 128fM, 32fM, 8fM, 2fM, 0.5fM, and 0.125fM. Six positive control genes must be order by concentrations from high to low.

See Also

positiveFactor

Examples

data(NanoStringData)
## obtain positive control genes
positiveControl(NanoStringData)

## assign a matrix
n=ncol(exprs(NanoStringData))
x=matrix(c(128,32,8,2,0.5,0.125)*80,ncol=1)
positive=matrix(rpois(6*n,x),ncol=n)
positiveControl(NanoStringData)=positive