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NanoStringQCPro (version 1.4.0)

assessHousekeeping,RccSet-method: assessHousekeeping

Description

Assess correlation and variance/variability of housekeeping genes

Usage

"assessHousekeeping"(rccSet, hk, covar, annotate = TRUE, plot = TRUE, digits = 2)

Arguments

rccSet
An RccSet object
hk
Either a boolean vector of length nrow(exprs(rccSet)) or a numeric vector of indices which genes in exprs(rccSet) are housekeeping genes
covar
character; colname in fData(rccSet) that can be used to label genes by a category of interest
annotate
Scalar boolean; if TRUE (default), probes will be "annotated" using the "GeneName" column in the fData(rccSet) slot
plot
Scalar boolean, plot pairwise relationships ?
digits
Scalar integer, the number of decimal places

Value

A dataframe with one row per housekeeping genes and several columns with metrics suggested to assess performance of defined housekeeping genes.

Details

Pairwise correlations of all defined housekeeping genes will be assessed and pairwise scatterplots will be generated. This function does not only output pairwise correlation coefficients, but also - for each housekeeping gene - the variance, the interquartile range (IQR) and median expression level across all samples in the experiment.