NanoStringQCPro (version 1.4.0)

posCtrlNorm,RccSet-method: Positive control normalization

Description

Applies positive control normalization to the data in an RccSet object.

Usage

"posCtrlNorm"(rccSet, summaryFunction = "sum", quietly = FALSE)

Arguments

rccSet

An RccSet object.

summaryFunction

Function to be used for the normalization (e.g. "mean", "median", or "sum"). User-defined functions similar to these can be specified here as well.

quietly

Logical. If TRUE, messages and warnings will not be shown.

Value

A copy of the input RccSet that has count data adjusted by positive control counts. The positive control scaling factor is recorded in PosFactor in the output's phenoData (if this column already exists in the input, it will be overwritten in the output copy).

Examples

data(example_rccSet)
pcnorm_example_rccSet <- posCtrlNorm(example_rccSet)