Osterberg at al. (2006) report growth phenotypes in yeast for a strain
collection over-expression ~600 C-terminal tagged integral membrane
proteins growth both under normal and three different stress
conditions.
Usage
data(OsterbergRaw)
Arguments
Format
OsterbergRaw is a 17 columns dataframe.
SYSTEMATIC
Systematic gene names of the studied membrane protein
COMMUN
Commun gene names of the studied membrane protein
TMHMM.C
The topology predicted by TMHMM (TransMembrane
prediction using Hidden Markov Models)
using the experimentally assigned C-terminal location for the protein as a
constraint. The topology is represented in the format Location of
N-terminus TMhelices Location of C-terminus (i and o stand for in
and out respectively)
WesternBlot
Protein expression levels (arbitrary units),
estimated from the band intensity and normalized to the internal
standard on each Western blot.
Bands
Proteins detected as two distinct bands with different
molecular mass on the Western blot analysis. category 1 indicates
that both bands were insensitive to Endo H digestion, 2 indicates
the higher molecular mass was shifted down upon Endo H digestion
on SDS/PAGE and one band was predominant compared to the other,
and 3 indicates that a higher molecular mass band shifted down
upon Endo H digestion on SDS/PAGE and both bands were equal
intensity on Western blot
Toxicity
Toxicity index from Spoko et al. (2006). The index
varies between 1 and 5, where 1 means the strain is dead, and 5
indicates no difference in growth rate compared with the wild type strain.
Phenotypes (significant or not) of over-expression
strains (LSCrate) in synthetic defined medium conditions
(Warringer et al., 2003). An average of two replicates is given.
Osterberg M, Kim H, Warringer J, Melen K, Blomberg A, von Heijne
G. (2006) Phenotypic effects of membrane protein overexpression in Saccharomyces
cerevisiae. PNAS. 103(30):11148-53. PMID: 16847257