build_injec_seq: Create Injection Sequence

Description

Create Injection Sequence

Create Injection Sequence from PlateObj (Single Plate)

Create Injection Sequence from MultiPlate (Multiple Plates)

Usage

build_injec_seq(
  plate,
  method,
  rep_DB = 2,
  rep_ISblank = 1,
  rep_suitability = 1,
  rep_blank = 2,
  repeat_std = 1,
  repeat_qc = 1,
  repeat_analyte = 1,
  repeat_dqc = 1,
  n_explore = 0,
  blank_after_top_conc = TRUE,
  blank_at_end = TRUE,
  blank_every_n = NULL,
  injec_vol,
  descr = "",
  prefix = Sys.Date(),
  suffix = "1",
  tray = 1,
  conc_df = NULL,
  grouped = TRUE
)
# S4 method for PlateObj
build_injec_seq(
  plate,
  method,
  rep_DB = 2,
  rep_ISblank = 1,
  rep_suitability = 1,
  rep_blank = 2,
  repeat_std = 1,
  repeat_qc = 2,
  repeat_analyte = 1,
  repeat_dqc = 1,
  n_explore = 0,
  blank_after_top_conc = TRUE,
  blank_at_end = TRUE,
  blank_every_n = NULL,
  injec_vol,
  descr = "",
  prefix = Sys.Date(),
  suffix = "1",
  tray = 1,
  conc_df = NULL,
  grouped = TRUE
)
# S4 method for MultiPlate
build_injec_seq(
  plate,
  method,
  rep_DB = 2,
  rep_ISblank = 1,
  rep_suitability = 1,
  rep_blank = 2,
  repeat_std = 1,
  repeat_qc = 1,
  repeat_analyte = 1,
  repeat_dqc = 1,
  n_explore = 0,
  blank_after_top_conc = TRUE,
  blank_at_end = TRUE,
  blank_every_n = NULL,
  injec_vol,
  descr = "",
  prefix = Sys.Date(),
  suffix = "1",
  tray = 1,
  conc_df = NULL,
  grouped = TRUE
)

Value

InjecListObj object

Arguments

plate: PlateObj object
method: choose method from database
rep_suitability: Number of re-injections for suitability vial.
repeat_std: number of re-injections for calibration standards. Default is 1.
repeat_qc: number of re-injections for QC wells. Default is 1
repeat_analyte: number of re-injections for unknown samples. Default is 1
n_explore: A number of exploratory samples to be injected at the top of the entire sequence. Default is 0
blank_after_top_conc: If TRUE, adding blank after high concentrations of standards and QCS.
blank_at_end: If True, adding blank at the end of queue.
blank_every_n: If no QCs, frequency of injecting blanks between analytes.
injec_vol: volume of injection in micro liters.
descr: Run description.
prefix: string at the beginning of the filename. Default is today's date.
suffix: string to be added to the end of the filename. Default is "1".
tray: Location in sample manager.
conc_df: data.frame matching compound name to a scaling factor. Maximum 20 compounds allowed.

Details

n_explore controls if exploratory samples are to be injected. A random sample from each CS and QC group will be sampled along with 1 blank sample.