# POD v1.2.0

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0th

Percentile

## Probability of Detection for Qualitative PCR Methods

This tool computes the probability of detection (POD) curve and the limit of detection (LOD), i.e. the number of copies of the target DNA sequence required to ensure a 95 % probability of detection (LOD95). Other quantiles of the LOD can be specified. This is a reimplementation of the mathematical-statistical modelling of the validation of qualitative polymerase chain reaction (PCR) methods within a single laboratory as provided by the commercial tool 'PROLab' <http://quodata.de/>. The modelling itself has been described by Uhlig et al. (2015) <doi:10.1007/s00769-015-1112-9>.

This is the R package 'POD'.

# Vignettes

A tutorial is provided for the usage of the Excel macro.

## Linux users

To get vignettes under Linux, enter options(browser="firefox"); browseVignettes("POD")

## Windows users

To get vignettes under Windows, enter options(browser="C:/Program Files (x86)/Google/Chrome/Application/chrome.exe") browseVignettes("POD") To use other browsers, adapt the path accordingly.

## Functions in POD

 Name Description print.pod Summary of POD objects foreign Support Other Platforms testdata Get Test Data computePOD Compute the Probability Of Detection (POD) analyzeSingleLab Analyze Single Lab Qualitative PCR Outcomes plotPOD Generate Plot to Analyze Single Lab PCR Outcomes No Results!