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Pasha (version 0.99.16)

Preprocessing of Aligned Sequences from HTS Analyses

Description

Pipeline and additional tools designed to handle aligned data from chromatin NGS experiments (less adapted for typical RNAseq applications). Specifically, starting from aligned reads after sequencing, the pipeline function aims at removing artefacts from datasets, estimate in-silico the fragments size, and generate WIG files with corrected values. The package also provides several tools for WIG files manipulations, scaling, merging and subtract (e.g. input).

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Version

Install

install.packages('Pasha')

Monthly Downloads

3

Version

0.99.16

License

GPL-3

Maintainer

Romain Fenouil

Last Published

August 1st, 2015

Functions in Pasha (0.99.16)

elongationEstimation

Shifting the positive strand vector and compute an overlap score to estimate the optimal elongation size for the fragments
.testFunctionalMultiread

Functions dedicated to the functional validation of multiread feature of Pasha package.
Pasha-package

Pasha package (Preprocessing of Aligned Sequences from HTS Analyses)
binVector

Divide a numeric vector in equal bins of signal
multiread_RemoveArtifact

Artifact Remover
mergeWigs

Merge several different wig files in a unique result file
pileupDouble

Piling vector of double
normAndSubtractWIG

Normalization and subtraction of WIG files
.testFunctional

Functions dedicated to the functional validation of Pasha package.
getArtefactsIndexes

Identify reads implied in piles potentially considered as PCR artefacts
processPipeline

A pipeline to accurately transform aligned reads to genomic enrichment scores
generatePiled

Pileup reads in a numeric vector with several piling and elongation options
readWIG and writeWIG

Read and Write genomic WIG (fixed step) files
WigRepeatAnalyzer

WIG Repeat Analyzer
AlignedData-class

Class AlignedData
multiread_UniformDispatch

Multiread scoring uniform dispatch
plotReadsRepartitionAnnotations

Graphical information about reads and annotations repartition
estimateElongationSize

Insert size estimation in-silico
multiread_CSEMDispatch

Multiread scoring CSEM dispatch