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ProActive (version 0.1.0)

sampleMetagenomePileup: sampleMetagenomePileup

Description

A subset of contigs from the raw whole-community fraction read coverage pileup file generated during read mapping. Report...

Usage

sampleMetagenomePileup

Arguments

Format

## `sampleMetagenomePileup` A data frame with 4,604 rows and 4 columns:

V1

Contig accession

V2

Mapped read coverage averaged over a 100 bp window size

V3

Starting position (bp) of each 100 bp window. Restarts from 0 at the start of each new contig.

V4

Starting position (bp) of each 100 bp window. Does NOT restart at the start of each new contig.

Details

This dataset was generated from a conventional mouse fecal homogenate. The whole-community extracted DNA was sequenced with Illumina (paired-end mode, 150 bp reads) after which the metagenome was assembled. The sequencing reads were mapped to the assembled contigs using BBMap. The bbmap.sh bincov parameter with covbinsize=100 was used to create a pileup file with 100 bp windows. A subset of 10 contigs from the pileup file were selected for this sample dataset. The contigs were chosen because their associated read coverage patterns exemplify ProActive's pattern-matching and characterization functionality across classifications: NODE_1911: elevation off left NODE_1583: elevation off right NODE_1884: gap off right NODE_1255: gap off left NODE_368: full gap NODE_617: elevation full NODE_1625: no pattern