FastQC-class: FastQC and associated classes
Description
The FastQC class stores results obtained from the FastQC application (see references
),
with a slot for each FastQC module. The SequenceQC class contains the QC results of a single lane of sequencing in three slots:
Unaligned
- FastQC results obtained from all reads (before alignment)
Aligned
- FastQC results obtained from only reads which aligned
Mismatches
- a data.frame
containing counts for the number of mismatches of each type found at each sequencing cycle
Slots of a FastQC object
Basic_Statistics
Per_base_sequence_quality
Per_sequence_quality_scores
Per_base_sequence_content
Per_base_GC_content
Per_sequence_GC_content
Per_base_N_content
Sequence_Length_Distribution
Sequence_Duplication_Levels
Overrepresented_sequences
Slots of a SequenceQC object
Unaligned
- FastQC results obtained from all reads (before alignment) Aligned
- FastQC results obtained from only reads which aligned Mismatches
- a data.frame
containing counts for the number of mismatches of each type found at each sequencing cycle MismatchTable
- a data.frame
containing counts of how many mismatches aligned sequences containReferences
FastQC - http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/