Replication profile for H.volcanii wild isolate DS2. Genomic DNA for deep sequencing was isolated from 100 ml culture in stationary phase (A650 > 1, DS2_stat sample) or 1 litre in exponential phase (A650 0.1, DS2_exp sample). Unique reads for the two samples were calculated in 1 kb genomic bins using ASM2568v1 genome assembly. The ratio was created by dividing 'score' values from replicating sample by non- replicating sample 'score' values, adjusted by total number of reads. The ratio values were further adjusted by multiplying them by 1.12 to put the values onto biologically relevant relative copy number scale from 1 to 2.
data(MFAseq)data frame with 3887 rows and 7 variables:
short chromosome name
left chromosome coordinate
right chromosome coordinate
replicating sample name
non-replicating sample name
ratio value in the current bin
adjustment factor used for the current ratio
Hawkins et al. (2013) Nature 503(7477):544-547 (PubMed)