Replication profiles for wild type and Dbf4-9myc samples (T7107 and T9394 strains). The cells were stained with DNA dye and sorted based on DNA content into S or G2/M phase fractions. Extracted DNA was sequenced and mapped to sacCer3 genome. Unique reads for replicating (S) and non-replicating (G2/M) samples were calculated in 1 kb genomic bins. The ratio was created by dividing 'score' values from replicating sample by non- replicating sample 'score' values, adjusted by total number of reads. The ratio values were further adjusted by multiplying them by 1.41 and 1.402 for wild type and Dbf4-9myc samples, respectively, to put the values onto biologically relevant relative copy number scale from 1 to 2. The relative copy number values were smoothed using cubic spline and compared using z score statistics.
data(sortSeq)data frame with 22696 rows and 10 variables:
short chromosome name
left chromosome coordinate
right chromosome coordinate
replicating sample name
non-replicating sample name
ratio value in the current bin
adjustment factor used for the current ratio
Group number of the current bin
Smoothed ratio value
Significance of ratio difference between Dbf4myc and W303 samples
Natsume et al. (2013) Mol Cell 50(5):661-74 (PubMed)