SGSeq (version 1.6.2)

assays: Accessing and replacing assay data

Description

Functions counts and FPKM are used to extract counts and FPKM values from SGFeatureCounts and SGVariantCounts objects. Function variantFreq is used to access relative usage estimates from SGVariantCounts objects.

Usage

FPKM(object, ...)
FPKM(object, ...) <- value
variantFreq(object)
variantFreq(object) <- value
## S3 method for class 'SGFeatureCounts':
counts(object)
## S3 method for class 'SGFeatureCounts':
counts(object) <- value
## S3 method for class 'SGFeatureCounts':
FPKM(object)
## S3 method for class 'SGFeatureCounts':
FPKM(object) <- value
## S3 method for class 'SGVariantCounts':
counts(object, ...)
## S3 method for class 'SGVariantCounts':
counts(object, ...) <- value
## S3 method for class 'SGVariantCounts':
FPKM(object, ...)
## S3 method for class 'SGVariantCounts':
variantFreq(object)
## S3 method for class 'SGVariantCounts':
variantFreq(object) <- value

Arguments

object

Object containing assay data

...

Arguments passed to method for SGVariantCounts objects. Argument option specifies whether the output should be based on the count of fragments compatible with the variant at the start (variant5p), end (variant3p) or either (variant5pOr3p) (the default), or whether output should be based on the count of fragments compatible with any variant belonging to the same event (event5p or event3p). Argument min_anchor specifies the minimum anchor length when computing FPKM values (defaults to 1).

value

Replacement value

Value

• Assay data for accessor functions or updated object for replacement functions.

Examples

x <- counts(sgfc_pred)
y <- FPKM(sgfc_pred)
u <- counts(sgvc_pred, option = "variant5p")
v <- FPKM(sgvc_pred, option = "variant5p")